Real-time Thermal PCR Cycler - qTOWER3 G touch
Location: A71-271
Our qTower3 G touch qPCR machine is a 96-well format and currently uses one SYBR Green module, however, can be equipped with up to six for multiplexing capabilities. One main function of this system will be to evaluate stress parameters on microbial communities collected from Arctic snow and ice samples.
Tecan Infinite M Plex
Location: A71-273
The Tecan Infinite M Plex is a multimode plate reader that is equipped with monochromator-based optics allowing free wavelength choice and scanning capabilities. This equipment can be used for all standard absorbance, fluorescence and luminescence assays.
Total Organic Carbon Analyzer (TOC-L-CPH)
Location: A71 - 274
The Shimadzu Total Organic Carbon Analyzer (TOC-L-CPH) with ASI-L Autosampler is used for the analysis of total carbon, total organic carbon and inorganic carbon to study the carbon content of snow, ice and sediments from environmental samples as well as their biological and geochemical transformation processes. Prior to analysis, water samples are prepared under the SafeFAST Classic Class II Biological Safety Cabinet and stored acidified (pH 2) in a refrigerator.
Optical Microscopes
Location: A71 - 211
Optical microscopes are available in the Microscopy Lab for different techniques, such as brightfield, polarization contrast, fluorescence. Microscopes available:
- Zeiss Stemi 305
- Zeiss Axio Scope.A1
- Zeiss Axiovert 5
- Zeiss Jenapol
- Leitz LaborLux 11 POL
The microscopes can also be equipped with the following cameras: Sony Alpha 6000 digital camera and Kern ODC-86 microscope camera.
We also have the FlowCam 5000 by Yokogawa, which is a particle analyzer capable of taking optical images. It is comparable to a flow cytometer and features a syringe pump and 100 x 700 µm glass capillary, where liquid passes through. During a sample run, images of the flow are taken with a camera, magnified with a 10x objective. Detected particles are compiled as images in a collage, which can then be grouped and classified by the user based on the particle properties (e.g. size description and morphology, among others) provided by the software. Sample volume ranges from 0.25 to 1 mL.
The FlowCam provides:
- fast data acquisition
- capability to distinguish various particles of the same size and morphology
- large sample volume compared to traditional methods like hemocytometry
- detailed statistics for number and volume of particles in environmental and laboratory samples.
The contact person for the FlowCam 5000 is Sven Peters.
